Which antibiotics could you use in your petri dishes to see if bacteria have taken in your plasmid? Why? Which antibiotics would you not use? Why?
You could use any, as long as there is so cells that have it inside. If there are none inside the bacteria, you would kill the thing. For example, if I used Kanamycin, and the bacteria wasn't resistant, then when I kill all other parts that aren't resistant to Kanamycin, so would the bacteria.
What are restriction enzymes and how do they work? Which one did you use and why?
Restriction enzymes are enzymes that attach to the DNA and make cuts on it to split the DNA. I used the "ECO R1" restriction enzyme since it was the only one that correctly split the DNA and the plasmid.
What would happen if you used an enzyme that cut the plasmid in two places?
What would happen if you used an enzyme that cut the plasmid in two places?
The plasmid would be missing a large chunk of code, which could be vital for the organism to survive. When I attach on the insulin gene from the Human DNA, then the recombinant DNA would be very small
How do you think this process is important in our everyday life?
How do you think this process is important in our everyday life?
This process could produce new parts, and create specific immunities to the bodies, and so much more.
How else could this process be used (be creative!) or search online to find current technologies using recombinant DNA.
How else could this process be used (be creative!) or search online to find current technologies using recombinant DNA.
There are many ways that this technology is being used today, such as medical testing or even genetically modified pets, like the GloFish.
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