Thursday, January 14, 2016

Recombinant Lab Report

In the lab we did, we recreated the process of producing recombinant DNA. When we first began the lab, we had cut out several colored pieces of paper. One color was the pieces that would create the plasmid, another color would be those that create the Human DNA and insulin gene, and the final color would represent the restriction enzymes. I had decided to tape together one piece that had the restriction site, and another that was "resistant" to kanamycin over the two other pieces that were resistant to tetracycline and amplicillin, which I discarded away. I taped together all the pieces that create Human DNA and the insulin gene, and sorted out the restriction enzymes, enzymes that attach to the DNA and make cuts on it. After testing all of the restriction enzymes on the model, there was only one that could make only one cut on the plasmid and two on the human DNA. I then cut out the insulin gene in the the human DNA along the restriction enzyme's path, and the same thing for the plasmid. We attach the parts together and we are done with the lab

Which antibiotics could you use in your petri dishes to see if bacteria have taken in your plasmid? Why? Which antibiotics would you not use? Why?
You could use any, as long as there is so cells that have it inside. If there are none inside the bacteria, you would kill the thing. For example, if I used Kanamycin, and the bacteria wasn't resistant, then when I kill all other parts that aren't resistant to Kanamycin, so would the bacteria.
What are restriction enzymes and how do they work? Which one did you use and why?
Restriction enzymes are enzymes that attach to the DNA and make cuts on it to split the DNA. I used the "ECO R1" restriction enzyme since it was the only one that correctly split the DNA and the plasmid.
What would happen if you used an enzyme that cut the plasmid in two places?
The plasmid would be missing a large chunk of code, which could be vital for the organism to survive. When I attach on the insulin gene from the Human DNA, then the recombinant DNA would be very small
How do you think this process is important in our everyday life?
This process could produce new parts, and create specific immunities to the bodies, and so much more.
How else could this process be used (be creative!) or search online to find current technologies using recombinant DNA.
There are many ways that this technology is being used today, such as medical testing or even genetically modified pets, like the GloFish.

http://rack.2.mshcdn.com/media/ZgkyMDEzLzA2LzEzLzU0L2RuYS40NjE1MC5qcGcKcAl0aHVtYgk5NTB4NTM0IwplCWpwZw/3b6190d2/275/dna.jpg

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